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BioChem Calculations

Simple Solutions to Numerical Biology & Chemistry

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Analyte Estimation using Spectrophotometric Methods & Excel

Beer law or Beer-Lambert’s law states that the absorbance of a solution is proportional to its concentration and pathlength. Mathematically,
Absorbance, A = e C L
And, A = e C L – Equation 1
where,
e = absorptivity at specified wavelength and temperature (M-1cm-1)
L = path length (in cm)
C = molar concentration of the solute

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